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豇豆花叶病毒衣壳蛋白编码区在中间组份的RNA核苷酸序列上的定位(英文)

MAPPING OF THE CODING REGIONS FOR THE CAPSID PROTEINS OF COWPEA MOSAIC VIRUS ON THE NUCLEOTIDE SEQUENCE OF MIDDLE COMPONENT RNA

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【作者】 Henk FranssenWim RoelofsenRob Goldbach

【Author】 Henk Franssen Wim Roelofsen Rob GoldbachReceived on November 4 , 1985Department of Molecular Biology,Agricultural University ,The Netherlands.Department of Microbiology,Agricultural University,The Netherlands.

【机构】 荷兰农业大学分子生物学系荷兰农业大学微生物学荷兰农业大学分子生物学系

【摘要】 对豇豆花叶病毒两个衣壳蛋白(VP37和VP23)的氨基端和羧基端氨基酸序列进行了分析,这些结果可以允许VP37和VP23编码区在病毒中间组份(M)RNA的核苷酸序列上进行基因定位。这两个编码区是相邻的,并表明,从M RNA的原始翻译产物中释出VP37和VP23的蛋白酶解部位,分别是谷氨酰胺-甲硫氨酸和谷氨酰胺-甘氨酸二肽序列。

【Abstract】 The amino-terminal and carboxy-terminal amino acid sequence of the two capsid proteins VP37 and VP23 of cowpea mosaic virus have been analysed. The results allow the mapping of the coding regions for VP37 and VP 23 on the nucleotide sequence of the middle-component (M) RNA. These regions are contiguous and indicate that the proteolytic cleavage sites used to release VP37 and VP23 from the primary translation products of M RNA are a glutamine-methionine and a glutamine-glycine dipeptide sequence, respectively.

  • 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,1986年01期
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