【摘要】 用核酸杂交法检测了酞丁安等三种化合物,对Ⅱ型单纯疱疹病毒DNA合成的影响。大约1μg HSV-Z DNA分子,用100μCi[α-³²P]-dCTP缺口转移作为探针,与硝酸纤维素滤膜上变性、固定的DNA点杂交。籍助放射自显影术或液体闪烁计数测定杂交程度,用以检测HSV-2DNA合成的水平。实验结果显示,酞丁安、阿克拉霉素A、B均明显抑制HSV-2 DNA的合成。50％抑制剂量分别为50.0μM,0.015μM与0.01μM。更多还原

【Abstract】 A nucleic acid hybridization technique for measurment of the inhibitory effect of Tai-Ding-An,Aclacinomycin A and B on herpes simplex virus type I DNA synthesis is described. Approximately 1 μg of the purified HSV-2 DNA molecules was nick-translated with 100 μM [α-32P}-dCTP.Theradiolabel-led HSV-2 DNA as probe was used for hybridization to DNA spots of virus infected cells with various concentration of added drug,which were denatured and fixed to nitrocellulose filters.The extent of hybridization was monitored by autoradiography or scintillation counting.The results revealed that Tai-Ding -An, Aclacinomycin A and B markedly inhibited HSV-2 DNA synthesis.The mean 50% inhibition dose values obtained were 50.0μM, 0.015μM and 0.0lμM,respectively.更多还原