【摘要】 本实验观察了Ⅰ型和Ⅱ型单纯疱疹病毒(简称HSV-1和HSV-2)的空斑特征和对温度的敏感性。病毒接种豚鼠胚细胞3天后,HSV-2形成的空斑较HSV-1的大,边缘也较整齐。两者对热(37℃水浴)灭活无明显差别,但HSV-2对低温(-70℃)的耐受性比HSV-1弱。HSV-1 DNA经EcoRI消化后,在我们的实验条件下,凝胶电泳可分辨出5条清晰的DNA带,而在此分子量范围内,HSV-2仅有两条带;用Hpa Ⅰ消化后,HSV-1有8条以上分辨清晰的带,HSV-2仅有一条可分辨的带。 本研究为HSV-1和HSV-2的分型鉴定、保藏提供了简便、准确的检测手段和实验依据。更多还原

【Abstract】 The plaque character and temperature sensitivity of herpes simplex virus type 1 ( HSV-1 ) and type 2 (HSV-2) were studied.The plaques formed by HSV-2 ( 3.27±0.33mm) in guinea pig embryo ( GPE ) cells were bigger than those by HSV-1 ( 1.79±0.24mm ) 3 days postinoculation. Also, the plaque margin of HSV-2 was neat,but that of HSV-1,non-neat. Although no difference of themosensitivity to 37℃ waterbath was found between HSV-1 and HSV-2, HSV-1 was more resistant to low temperature storage (-70℃) than HSV-2.After EcoRI digestion, HSV-1 DNA showed five well-separated bands and only two HSV-2 DNA bands were found in the electrophoretic patterns. Moreover, over eight well-separated bands were shown in the HpaⅠ cleaved HSV-1 DNA,whereas only one band was clearly visible in the HapⅠ cleaved HSV-2 DNA.更多还原