【摘要】 本文报道用醋酸棉酚处理体外人食管癌细胞(Eca—109)后,细胞的生长、分裂、再繁殖能力和DNA合成的变化。结果表明,醋酸棉酚对Eca—109细胞具有较强杀伤作用。棉酚20μg/ml作用48小时可明显抑制细胞的生长和分裂,并使细胞完全丧失再繁殖能力。棉酚对细胞形态的损伤主要表现为胞质空泡化和核固缩。棉酚作用72小时,对细胞DNA合成的抑制率为92％。本文还就棉酚对食管癌细胞杀伤作用的机理进行了讨论。更多还原

【Abstract】 The purpose of present study was designed to investigatethe efferts of Gossypol acetic acid on Eca-109 human esophageal cancer cell 1ine.The results showed that Gossypol acetic acid could exert its cytotoxic effect. At the concentration of 20μg/ml for 48 hours the cell growth and mitosis were inhibited significantly as shown by cell growth curve and mitosis index, and finally the cells completely lost their proliferating ability as confirmed by colony formation test. The morphological appearance of the cell after treatment with gossypol was mainly of cytoplasmie voculation and nuclear pyknosis. After exposure the cells to Gossypol acetic acid for 72hours the DNA synthesis of the cells was inhibited considerably. The rate of inhibition was 92 per cent. The mechanism of gossypol action was discussed briefly.更多还原