【摘要】 应用本室建立的胎盘蛋白(PP)酶联免疫吸附试验(PP—ELISA)方法测定了正常人、孕妇、肿瘤病人和老年病人中的PP相对含量。结果发现:(1)正常人和孕妇血清的PP水平很低,OD的均值和标准差(X±SD)分别为0.051±0.025和0.07±0.035;(2)检查的肿瘤病人中PP阳性(>X+3SD)的例数如下:直肠癌3/6,卵巢肿瘤1/4,乳腺癌4/11,肺癌7/29,胃癌4/13,肝癌10/19,食道癌3/8,鼻咽癌3/8,纵隔瘤2/2,其他肿瘤6/20;(3)在302例老年病人中PP水平升高者共9例,其中1例为直肠癌,2例死于肝硬变,2例怀疑为肿瘤。以上结果提示,PP水平的检测可能有助于临床诊断和判断预后。此外,我们对PP抗原成分进行了初步分析,证明它不是AFP,r—GT或HCG。PP抗原凝胶过滤行为表明它至少含有三种或三种以上成分。更多还原

【Abstract】 The concentration of placental proteins (pp) in the serumof normal subjects, pregnant women, malignant tumor patients and elderly patients was examined by specific engyme -linked - lmmunoabsorption assay (pp -ELISA). The results are as follows:1) the levels of pp in normal subjects and pregnant women were relatively loow, the optical density are 0.05±0.025 and 0.07±0.035 respectively, 2) pp-positive (oD >0.13) occurred in. serum from 3 out of 6 cases of colerectal cancer, 1 out of 4 cases of ovarian cancer, 4 out of 11 cases of breast cancer, 7 out of 29 cases of lung cancer, 4 out of 13 cases of stomach cancer, 10 out of 19 cases of liver cancer, 3 out of 8 cases of rhinophanynx cancer, 3 out of 8 cases of Desophageal carcinoma,2 out of 2 cases of. mediastinal tumor and 6 out of 20 cases of miscellaneous cancer; 3) pp-positive occurred in the serum from 9 out of 302 cases of elderly patients In these 9 cases, 2 cases had died, 1 case was diagnosed as colorec ?tal cancer, 2 cases were tumor under suspicion. This results suggested that pp level might be of help in diagnosis and prognosis of cancer.In addition,the component of pp antigen was analysed by sephadex G150 gel filtration. OUT preliminary experiment shows s that there are three or more componts in pp antigen.更多还原