【摘要】 本文以3—甲基胆蒽致小白鼠纤维肉瘤后,选其高度恶性的三个不同细胞株作为研究对象。每株又分别加上补体和10％含有抗B型血抗原之抗体的人血清,杀灭层粘连蛋白阳性之细胞后,培养其能活存之细胞,分别建立另外三种低度恶性的细胞株,其细胞表面缺乏层粘连蛋白样物质。上述所有细胞株用改良的Boyden室和琼脂糖滴进行体外移动性的研究。 结果指出;层粘连蛋白阳性细胞株的移动性远远大于层粘连蛋白阴性之细胞株。当培养基内加上外源性的层粘连蛋白时,层粘连蛋白阴性的细胞株则明显地增加了其移动性。事实上,本文以及Varani等的工作均证明了层粘连蛋白阳性瘤细胞株具有高度恶性和高转移率。反之,层粘连蛋白阴性之细胞株,不但恶性度低而且基本上没有转移。更多还原

【Abstract】 Several variant cell lines which are deficient in surfacelaminin have been isolated from heterogeneous murine tumor populations. The parent populations from which these variant lines were isolated contain cells which express high levels of surface laminin as indicated by immunof luore ?scence, immunoperoxidase/electron microscopy and immunoprecipitation. The laminin-deficient lines were compared to their respective parent populations for motility in both the Boyden chamber assay and the agarose assay. In both assays, the laminin-positive populations were much more motile than the laminin-deficient lines. The addition of exogenous laminin to the laminin ?deficient lines significantly increased their motility. These observation are of interest since cell motility is thought to contribute to tumor cell metastasis, and the laminin-positive cell populations are highly tumorigenic and metastatic while the laminin-def icient cells are of low tumorigenicity and are virtually non-metastatic.更多还原