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淫羊藿注射液对试管内鸡胚股骨生长的促进作用

The Stimulative Effect of Injection Epimedium on the Growth of Chick Embryo Femur in Vitro

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【作者】 高子范; 杨宗智; 马克昌; 刘月桂; 刘鲜茹; 刘万智; 李鸿超; 张灵菊;

【Author】 Gao Zifan Institute of Orthopedics,Luoyang,

【机构】 河南省洛阳正骨研究所; 河南省洛阳正骨研究所;

【摘要】 应用鸡胚股骨试管内培养的方法,研究了淫羊藿注射液对鸡胚股骨生长的促进作用。9天鸡胚股骨培养6天后,用药组股骨的长度、干重和。35S 的掺入强度均较对照组明显增加,分别为对照组的116%、112%和133%,两组比较差异非常显著(P<0.01)。证明淫羊蓉注射液对鸡胚股骨的生长和鸡胚股骨蛋白多糖的合成活性有明显促进作用。所用药液适宜浓度为2:100~3:100(淫羊霍注射液:培养液)。

【Abstract】 This is astudy of the effect of injection Epimedium on thogrowth of chick embryo femur in vitro.Nine-day-old chick embryo femora were cultivated with the roller-tube culture method for 6 days,the length,dry-weight and 35S- sulfate uptake rate of the Epimedium-treated femora incased by 116%,112% and 133% respectively in comparison with corresponding values of the control.The marked differences of the two groups have proved that the injection Epimedinm markedly promoted the growth of the chick embryo femora and their protein pulysaccharide synthesis in vitro.The results showed that the ideal proportion of the injection to medium was 2:100~3:100.The injection used in our experiments was a compound,the effective elements of which are to be isolated and identified.

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