【摘要】 <正> 1983年作者等发现肝癌能引起宿主红细胞钠泵活性升高,其机理尚不清楚。据Yeagle报道膜的胆固醇含量减少可增加红细胞膜钠泵的活性及转运能力。Owen等证明,胆固醇增加能降低红细胞膜的流动性。荧光偏振技术是研究细胞膜脂流动性的重要方法之一,偏振测量的灵敏度高,重复更多还原

【Abstract】 In our previous reports, the activity of erythrocyte sodium pump was found to beincreased in hepatoma bearing mice, and the abnormal lipid composition of the membranein patients with liver cancer was found to be associated with the change of fluidity oferythrocyte membrane. Moreover, the incressed membrane fluidity may raise the activityof sodium pump, This may be related to the mechanism of the increase of sodium pumpactivity. In this study the membrane fluidity was examined by the method of measuringthe degree of fluorescence polarization of the erythrocyte membrane using DPH as thefluorescent probe in Hep A ascitic hepatoma bearing mice. The results showed that thefluorescence polarization (P) of the normal group was 0. 287±0. 003, the microviscosity(η) was 3.338±0.099; and the P value of tumor group was 0.271± 0 .003, ηwas 2.897±0. 083(n = 19, p<0.01), indicating an increase of membrane fluidity. Thus, the increasederthrocyte membrane fluidity may closely relate to the increase of erythrocyte sodiumpump activity in tumor bearing host.更多还原