【摘要】 <正> 微核试验是检测环境与工业化学诱变物引起细胞遗传学损伤的简易而敏感的方法,其中小鼠骨髓微核试验已列为遗传毒理学常规致突变性检测方法之一。恰当的吉氏染色虽可提高多染红细胞(PCE)与正染红细胞(NCE)的分辩力,但遇与微核色泽、形态、大小相似的RNA包涵体或其他物质时,常不易识别而影响计数。本文应用吖啶橙荧光染色方法,主要在小鼠微核试验中进行了尝试。比吉氏(Giemsa)染色法简便、快速、更多还原

【Abstract】 The micronucleus assay has been widely used in vivo as a cytogenetic test for detec-tion of environmental and industrial chemicals that have clastogenic activities. Giemsastaining has been routinely used for the test, but it was difficult to distinguish true micro-nuclei from basophilic granules. Acridine orange metachromatic fluorochrome differentia-tes DNA (yellowish green) from RNA (red-orange) sharply. Micronuclei emit brightyellowish green fluorescence and were easily distinguished from RNA containing inclu-sions and cytoplasmic granules in various cells. Thus, this technique is very specific forrecognization and enumeration of micronuclei. The advantage of acridine orange fluo-rescent technique was proved by comparison with Giemsa staining of the same cells andtriple enumeration of micronuclei induced in mouse bone-marrow cells by four dose levelsof mitomycin C of the two methods. Because of its relative accuracy and ease of executionthe acridine orange fluorescent technique is recommended in micronucleus study.更多还原