【摘要】 <正> 近三年内已有报道从10余种人体恶性肿瘤应用DNA转染和分子生物学技术鉴定和分离癌基因,例如从膀胱癌、肺癌分离出Ha-ras;从肺癌、结肠癌、胆囊癌、胰腺癌卵巢癌、某些软组织肉瘤中分离出Ki-ras;从纤维肉瘤、横纹肌肉瘤、神经母细胞瘤、髓细胞性白血病和T细胞淋巴瘤等分离出N-ras等。但关于我国常见的原发性肝癌的转化基因尚未见正式报道。本文描述从原发性肝癌、肝癌7402细更多还原

【Abstract】 The transfaction of NIH 3T3 mouse cell was performed with DNAs from humanprimary hepatocellular carcinoma （PHC） and Hepatoma 7402 cell line. Human N-ras gene（7.2 and 9.0Kb EcoRI bands） was identified in all the transformants from PHC and 7402DNA. Ha-ras gene was also observed as a 6. 6Kb BamHI band in transformed cells from7402 cell DNA, but absent in transformants from PHC DNA. The Poly （A）⁺ mRNA was isolated from 9 PHC, 1 tumor-adjacent liver and 1 normalliver samples. Northern transfer and hybridization indicated that the N-ras gene wasover-expressed in 6 out of 9 PHC whereas no detectable expression for Ha-ras or Ki-rasgenes. The protein synthesis of 7402 cell line and transformants from 7402 and PHC DNAwas analyzed by ³⁵S-methionine incorporation, Anti-p21 antibody immunoprecipitation,protein A absorption, PAGE and autoradiography. The results indicated that p21synthesized was remarkably enchanced in 7402 cell and transformed NIH 3T3 cell ascompared with untransformed cell. Taking together with the data presented it stronglyimplies that N-ras gene is one of the transforming gene for human liver cancer.更多还原