【摘要】 本文用间接免疫荧光技术筛选出一株具有相对肺腺癌特异性的McAb LC85a,其与2例人肺腺癌细胞有规则的线性膜荧光反应,与胎儿胰腺间质、正常成人胰腺、肝脏、胰腺癌间质及某些结肠癌、卵巢癌组织、宫颈癌CC-801、鼻咽癌CNE细胞株有交叉反应,但为不规则的细胞间质荧光反应,与肺鳞癌、胃癌等11种肿瘤细胞株均无反应。此种膜抗原荧光反应可资鉴别肺鳞,腺癌.更多还原

【Abstract】 One set of monoclonal antibody has been produced in Balb/C mouse against primary human lung adenocarcinoma obtained from surgical biopsies by immunofluorescent assay. The monoclonal antibody designated LC85a was identified after screening with immunofluorescent assay of the hybridoma cells obtained from immunizing mouse with lung adenocarci-noma cell line LTEP-a-2.The antibody did not react with homologous B and T lymphocytes, normal human glandular tissues, 3 non-lung glardular tissues, fibroblasts or erythrocytes as well as fetal lung, gastric, colonic and renal tissues. The antibody was further characterized with immunofluorescent staining of fresh cryostatic of 8 non-lung human tumors and squamous carcinoma of lung as well as 9 non-lung cancer cell lines and lung squamous or small cell carcinoma cell lines. All showed negative staining reactions. It was found that this McAb reacted only with the cancer cell membrane in 1 of 4 cases of adenocarcinoma of colon and 1 of 3 cases of ovary cancer and intercellular substances of both fetal pancreatic tissues and human panceatic adenocarcinoma as well as liver tissue. Negative anti-CEA, anti-αFP and anti-E receptor blockade staining tests showed the McAb was neither against GEA,αFP nor E receptor. Thus, our data suggested that this McAb was specific for human mucus adenocarcinoma. Since 2 out of 4 clinical lung adenocarcinoma biopsies tested showed positive reactions with this monoclonal antibody, therefore it may prove its value in clinical differential diagrosis as well as staining of doubtful adenocarcinoma更多还原