【摘要】 在Ⅲ型Polio病毒McAb的研究中,用ELISA法筛选出60株阳性杂交瘤细胞系,其中11株中和试验阳性。ELISA试验型特异的McAb有10个,中和试验型特异的McAb有5个。McAb C₃₆和C₅₀只能与疫苗株和类疫苗株起中和反应而不能与非类疫苗株病毒起反应。因而这二株McAb具有区分Ⅲ型Polio野毒株与疫苗株相关病毒株的能力。另外应用McAb初步分析了17株Polio Ⅲ型病毒的抗原性。更多还原

【Abstract】 Sixty mouse hybridoma cell lines secreting antibodies against type 3333333 polio-viruses were established by fusion of a mouse myeloma cell line SP2/0 with spleen cells from mice immunized with Saukett and Zhong Ⅲ2 virus strains. Comparison of microneutralization test (MNT) , immunofluorescent test (IF) and ELISA for screening positive hybridoma cell lines was carried out. ELISA gives the highest positive rate(30.4% ) while the positive rates of MNT and IF are 15.6% and 11.6% respectively.Seventeen type Ⅲ wild polioviruses and Zhong Ⅲ2 as well as Saukett virus strains were tested with 15 McAbs against type Ⅲ poliovirus by cross-ELISA. Six "subtypes" of type Ⅲ poliovirus were proposed. They are vaccine, vaccine-like, intermediate, Saukett, Saukett-like and approximate Saukett strains.Among 11 hybridomas secreting McAb possessing virus-neutralizing activity, 2(C50, C36 ) neutralized Zhong Ⅲ2 vaccine virus and three vaccine-like strains in MNT. However, they did not neutralize Saukett and other 2 non-vaccine-like strains. So we suggest that McAb C50 and C36 may be valuable for distinguishing wild type Ⅲviruses from those of vaccine related strains.更多还原