【摘要】 <正> 棉酚抗生育作用业已被国内外学者所证实(1～4)。并对其作用部位、机理、代谢、毒性等进行了深入地研究[5]。分子及酶水平的分析表明,棉酚可抑制三羧酸循环中数种酶的活性[6,7]。Lee等[8]认为棉酚是LDH-X的一种选择性抑制剂。另一方面,有学者[9]证明磺胺类药物亦具有杀伤精子的作用。我们初步实验也证实磺胺嘧啶(SD)可抑制人精子LDH-X活性及对精子有直接杀伤作用。本研究旨在观察在体外条件下醋酸棉酚与SD是否具有抑制人精子LDH-X与杀伤精子的协同作用。现将实验结果报道如下。更多还原

【Abstract】 Sperm marker enzyme LDH-x was extracted from fresh sperms and its activity Was determined as incubated respectively with gossypol acetate, sulphadiazine (SD) and gossypol acetate plus SD for 15 minutes. Furthermore, sperms viability following capacitation and treatment with the above chemicals was also assayed. The results showed although that both the drugs were effective to inhibit the activity as well as human sperm viability, yet the synergic effects of the drugs seemed to be stronger更多还原