【摘要】 从龙胆试管苗的叶肉细胞分离得原生质体。培养4—5天后能见到第一次分裂,一个月后形成肉眼可见的小愈伤组织。试管苗的低温预处理是龙胆叶肉原生质体能否分裂的重要条件;似乎只有体积小的原生质体能保持持续分裂。获得的愈伤组织正在诱导器官分化。更多还原

【Abstract】 Protoplasts were isolated from mesophyll cells of shoot cultures of gen- tian (Gentiana scabra Bunge).First division occurs after 4—5 days in culturing and small calli are visible to the naked eye within one month.Low temperature pretreat- ment of source materials is the important factor in connection with the division of mesophyll protoplasts of gentian.It seems that sustain division is within the limits of the protoplasts of small size only.Calli derived from protoplasts are being diffe- rentiated.更多还原