【摘要】 昆虫寄生性线虫的研究是个新的领域,在国内外是生防中的一个新课题。本文报道了我国自澳大利亚引进新线虫后,采用经高压灭菌的大蜡螟老熟幼虫置于营养琼脂斜面上培养线虫种,克服了国外沿用以动物内脏为主要原料保种、连续饲养线虫,导致线虫生活力衰退及容易污染问题,简化了培养基的制备、接种条件及手续,使线虫种的培养可以在一般微生物实验条件下进行。同时,介绍了大量繁殖线虫的新方法。试验筛选出应用国内容易获得的以淀粉类为主要原料的物质,作为培养线虫的具体培养基配方,简化了国外分别培养共生菌种及线虫种等操作手续,为新线虫作为生物防治材料打下了基础。更多还原

【Abstract】 This paper reports the use of sterilised larva of Galleria mellonlla to rear Neoaplectana spp.introduced from Australia.Using this method,it may solve problem of pollution and decaying of nematodes cultured on animal liver heart media.The technique is simple and can be used in any general microbiological laboratory. A new method of mass production of nematodes is also reported. By which nematodes of Neoaplectana can be produced in amylaeeous ma- terials without inoculation of symbiotic bacteria before experiment.更多还原