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间接免疫过氧化物酶试验诊断阿米巴病

IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY

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【作者】 陈雅棠; 刘约翰; 王其南; 马映雪; 余登高; 郭增柱; 王正仪; 桂淑环;

【Author】 Department of Infectious Diseases, First Hospital: Chen Yatang, et al

【机构】 重庆医学院第一医院传染病学教研室; 北京热带医学研究所; 北京热带医学研究所;

【摘要】 用间接免疫过氧化物酶试验(ⅡP)对15例急性阿米巴病人血清特异性抗体进行了检测,12例阿米巴肝脓肿病人全部阳性,滴度分布为1:40~1:640;3例急性阿米巴痢疾病人亦全部阳性,但滴度较低,为1:40~1:160。本法与间接荧光抗体试验和酶联免疫吸附试验相比,阳性率无显著差异。ⅡP操作简单,不须荧光显微镜及阿米巴纯培养,有较好的特异性,故适于普通实验室用于阿米巴病的诊断。

【Abstract】 The indirect immunoperoxidase assay (IIP) was evaluated for the detection of specific antibodies in sera from 15 cases of acute atriebiasis. 12 cases of amebic liver abscess were all positive for IIP assay, the titres ranged from 1:40 to 1 = 640; 3 cases of acute amebic dysentery were also positive for IIP assay, but their titres were comparatively lower and ranged from 1:40 to 1:160. Compared with the results of IFA and ELISA, no significant difference was found. IIP assay is easy to perform, does not require fluorescent microscope and amebic axenic culture.and has good specifity. Therefore, this method can be used for the diagnosis of acute amebiasis in a general laboratory.

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