【摘要】 <正> 作者曾报告,用~3H-TdR掺入法比较32份人脐血对不同种属的TF(TF-h及TF-P)的反应,并进一步比较了不同浓度的TF-h和TF-P对PHA和ConA诱导的脐血淋转的影响。结果说明,脐血T细胞对TF有着复杂的反应性,而且经不同浓度稀释之后,原先对PHA或ConA诱导的淋转发生抑制反应的标本,表现了淋转的恢复或促进。更多还原

【Abstract】 Porcine transfer factor（TF-P） was prepared from porcine spleen by means of hom-ogenization and vaccum dialysis. Fractionized by Sephadex G-10 gel chromatography,TF-P could be separated into seven fractions but five fractions were obtained from human transfer factor（TF-h） .These fractions of TF-P and TF-h and different dilutionsof TF-P were tested for biological activity in vitro. By the use of ³H-TdR uptake assay we found that, both of whole TF-P and TF-hpossessed suppresivs activity to PHA-induced human periperal blood lymphocyte tran-sformation. Whereas, Fraction Ⅲ of TF-P and Fraction Ⅲ and Ⅳ of TF-h containedcomponents responsible for augmentation of PHA-induced responses. When TF-P was diluted 10^-2 to 10^-5 and cultured with normal human peripheralblood lymphocytes, we found that the original suppression effects of whole TF-P toPHA-induced lymphocyte transformation were converted to normal. The optimum dil-ution of TF-P was 10^-3, which were shown to augment responses. It shoud be notedthat the responsiveness of normal human lymphocytes to TF-P was dose dependent.更多还原