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不同组分及不同浓度的猪转移因子(TF-P)对正常人淋转的影响

Effects of Different Dilutions and Fractions of TF-P on Normal Human Lymphocyte Transformation

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【作者】 李在连岳启安赵淑梅马威丽

【Author】 Li Zai-lian;Yue Qian;Zhao Shu-mei;Ma Wei-li Department of Microbiology, Chang wei Medical College

【机构】 昌潍医学院微生物学教研室昌潍医学院微生物学教研室 潍坊潍坊潍坊

【摘要】 <正> 作者曾报告,用~3H-TdR掺入法比较32份人脐血对不同种属的TF(TF-h及TF-P)的反应,并进一步比较了不同浓度的TF-h和TF-P对PHA和ConA诱导的脐血淋转的影响。结果说明,脐血T细胞对TF有着复杂的反应性,而且经不同浓度稀释之后,原先对PHA或ConA诱导的淋转发生抑制反应的标本,表现了淋转的恢复或促进。

【Abstract】 Porcine transfer factor(TF-P) was prepared from porcine spleen by means of hom-ogenization and vaccum dialysis. Fractionized by Sephadex G-10 gel chromatography,TF-P could be separated into seven fractions but five fractions were obtained from human transfer factor(TF-h) .These fractions of TF-P and TF-h and different dilutionsof TF-P were tested for biological activity in vitro. By the use of 3H-TdR uptake assay we found that, both of whole TF-P and TF-hpossessed suppresivs activity to PHA-induced human periperal blood lymphocyte tran-sformation. Whereas, Fraction Ⅲ of TF-P and Fraction Ⅲ and Ⅳ of TF-h containedcomponents responsible for augmentation of PHA-induced responses. When TF-P was diluted 10-2 to 10-5 and cultured with normal human peripheralblood lymphocytes, we found that the original suppression effects of whole TF-P toPHA-induced lymphocyte transformation were converted to normal. The optimum dil-ution of TF-P was 10-3, which were shown to augment responses. It shoud be notedthat the responsiveness of normal human lymphocytes to TF-P was dose dependent.

【基金】 中国科学院科学基金
  • 【文献出处】 中国免疫学杂志 ,Chinese Journal of Immunology , 编辑部邮箱 ,1985年06期
  • 【被引频次】5
  • 【下载频次】21
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