【摘要】 <正> IgE是人血清中含量最少的一类免疫球蛋白,目前多用放射免疫或酶免疫技术作定量测定。Ceska等对纸片放射免疫吸附试验（PRIST）方法作了详述。此法简便、快速、敏感、特异。PRIST是一种因相双抗体夹心技术,先将抗IgE抗体偶联于溴化氰活化的滤纸片上,使与待测标本或IgE工作标准进行反应,洗净后加入¹²⁵I—抗IgE抗体再进行反应,洗净后测定滤纸片上复合物的放射能。后者随IgE量增多而增强。我们在建立PRIST方法之后,将其应用于检测支气管哮喘病人血清总IgE。现报告如下。材料与方法更多还原

【Abstract】 This paper describes the method of paper radioimmunosorbent test (PRIST)and its clinicaapplication of determination of total serum IgE in bronchial asthmatics. The sensitivity ofthis method was about 1 IU/ml and reproduclbility, accuracy and specificity were identified.The geometric means of total serum IgE levels of healthy donors, atopic asthmatics, mixedtypeasthmatics and infective asthmatics groups were 459.89, 716.79, 755 .26 and 537.30 IU/ml respective-1y. The correlation coefficient for agreement between PRIST and enzyme-linked immunosorbentassay(ELISA)was 0. 92(P<0.001). The advantages of PRIST were relatively simple, rapid, sensi-tive and specific.更多还原