【摘要】 五年来,通过对湖羊精液冷冻稀释液、冷冻工艺、输精方法等多项试验,已选出一套技术程序.冻精活力达0.45.在37℃条件下,精子生存时间10.6小时,生存指数3.33,谷草转氡酶(GOT)含量2.33单位/亿,呼吸强度(耗氧量)7.768微升/亿.在农村进行了1416头羊的试验,受胎率逐年提高,情期受胎率41.8%.1983年最高组合达75%(42/56).用1.5M DMSO/PBS冷冻保存液,对43枚湖羊早期胚胎进行冷冻并在液氮里保存.解冻34枚胚胎有24枚正常(占70.58%),其中10枚胚胎用外科手术法移植给3头受体母羊,2头怀孕(占66.6%),共产活羔3头.更多还原

【Abstract】 AbstractThe following main items were studied: selection of the diluents for frozen semen of Huyang sheep, freezing technique and insemination method, which resulted in obtaning an effective process during 1979～1983.The conception rates of tested ewe was increased from 29.18 to 41.8% by the insemination of 1416 sheep during an estrus period. The highest conception rate of tested group was above 75%(42/56) in 1983.43 early-to-fully-expanded-blastocysts were placed in 1.5 M DMSO/PBS solution, frozen and then stored in liquid nitrogen. After thawing, 34 embryos were recovered and 24(70.58%) were judged as normal.更多还原