【摘要】 本文报道了用人Rh（+）红细胞免疫BALB/C小鼠,取其脾细胞与骨體瘤细胞系NS/融合,获得了一株能分泌抗人A型红细胞单克隆抗体的杂交瘤细胞株,定名为ZMC9。血凝试验结果表明,ZMC₉具有较好的特异性。经临床548例血型测定,未见交叉反应。该杂交瘤细胞株经组织培养传代四个月,分泌抗人A型红细胞的性能稳定。注入BALB/C小鼠腹腔产生含高滴度（1∶262.144）的腹水及肿瘤。更多还原

【Abstract】 In this paper, we report our achievements in the estabilishment of one hybridoma cell lines secreting anti-human group A erythrocyte monoelonal antibodies（MC Ab）by means of fusion between mouse myeloma cell NS1 and spleen cells from BALB/C mice immunized withhuman group ARh（+） erythrocyte. The bybridoma cell lines have been named ZMC₉. The result of blood-agglutination test showed that MC Ab secrted by ZMC₉ was of specificity. No Cross reaction was detected in 548 cases of blood sample. ZMC₉ has been proved to be in seeretion of MC Ab in tissue culture for 4 months. Inoculation and transplanted ation of ZMC₉ into BALB/C mice could produce ascitic fluids with high titer （1: 262144） and solid tumors.更多还原