【摘要】 我们用以下方法研究了胺菊酯（合成拟除虫菊酯）的遗传毒性:①非程序性DNA合成（UDS）检测胺菊酯对FL细胞DNA的损伤作用;②平板掺入试验及波动试验检测由胺菊酯诱发的鼠伤寒沙门氏菌的回复突变。结果:①胺菊酯浓度5×10^-2～5×10⁰μg/ml,不论检测系统中是否加入S9混合物均诱发FL细胞的UDS,并呈剂量反应关系;②在平板试验中,如检测系统中不加S9混合物,胺菊酯（5、50及500μg/皿）不诱发TA100、TA98及TA97的回复突变;加入S9混合物,胺菊酯（500μg/皿）使TA100及TA97的回度菌落数增加至对照的两倍左右;③在波动试验中,检测系统不论是否加进S9混合物,胺菊酯（50及500μg/ml）诱发的TA97的阳性管数均显著高于对照管。上述结果证实胺菊酯对哺乳类细胞的DNA有损伤作用并对鼠伤寒沙门氏菌有诱变性,我们认为,如将UDS检测与Ames试验结合,必要时辅以波动试验,可提高对致突变性和致癌性化合物在初筛中的检测价值。更多还原

【Abstract】 ine genototoxierty of tetrametnm has oeen studica by means of UDS assay to measure its DNA-damaging ability in FL line of human amnion cells, and plate incorporation assay and fluctuation test to estmate its mutagenicity in S. typhimurium. Whether S9 mix is added or not, the tetramethrin (5×10^-2～5×10⁰μg/ml) has been proved to be able to induce UDS in EL cells with a dose-response effect found. In the plate incorporation assay, no mutagenic activity can be found in S. typhimurium strains of TA100, TA98 and TA97 exposed to tetramethrin （5, 50 and 500 μg/plate） without S9 mix. However, in the presence of 59 mix, tetramethrin （500μg/plate） induces His⁺ revertants of S. typhimurium TA100 and TA98, nearly double that of the control. While, in the fluctuation test, irrespective of the adding of S9 mix, the number of tubes positive of TA97 induced by tetramethrin （50 and 500 μg/ml） increases more markedly than that of the control. These results indicate that the tetramethrin used in this study is DNA-damaging to mammalian cells and mutagenic to S. typhjmurium. Tile authors believe that the combination of UDS assay with Ames test, supplemented with the fluctuation test if necessary, would give a high value of sensitivity in screening mutagenic/carcinogenic chemicals.更多还原