【摘要】 用弓形虫速殖子可溶性抗原对BALB/c小鼠免疫所得脾细胞与NS-1骨髓瘤细胞融合,获分泌抗弓形虫抗体阳性杂交瘤11个,阳性率为5.1％。选择强阳性杂交瘤1个进行克隆化培养,获得1株稳定分泌特异单克隆抗体杂交瘤细胞株。ELISA测试该株杂交瘤细胞接种BALB/c小鼠诱生腹水抗体效价为1:51,200。此株分泌抗弓形虫单克隆抗体鉴定属IgG_2a亚型。更多还原

【Abstract】 Splenocytes from BALB/c mice immunized with soluble antigen of Toxoplasma gondii tachyzoites were fused with the NS-1 mouse myeloma cells. 11 hybridomas secreting antibodies against T.gondii were obtained,the positive rate being 5.1%.Among the 11 hybridomas, one strong antibody secreting strain was selected, and through cloning 5 times, a hybridoma cell line was developed, secreting steadily the monoclonal antibody (McAb) with specificity for T. gondii. ELISA test showed that the titer of the McAb in the ascites fluid from the BALB/c mice injected with the hybridoma cells was as high as 1:51,200. It was confirmed that the McAb belonged to IgG2a.更多还原