【摘要】 作者曾进行日本血吸虫虫卵可溶性粗虫卵抗原(SEA_j)的血清学抗原分析,并用亲和层析法从粗SEA_j中分离获得主要血清学抗原(MSA)。本文进一步用酶联免疫吸附试验(ELISA)比较这两种抗原的血清学活性。结果表明,两种抗原与轻流行区血吸虫病人的粪检阳性符合率均为91.67％。SEA_j组的假阳性率为1.2％,MSA组则未出现假阳性。经棋盘滴定,SEA_j的最适抗原浓度为2.5μg/ml,MSA为1μg/ml。前者的抗原用量为后者的2.5倍,说明MSA具有较高的血清学活性。更多还原

【Abstract】 The major serological antigen (MSA) involved in crude soluble egg antigen of S. japonicum (SEAj) has been isolated from the crude one by affinity chromatography and characterized by electrophoresis in previous studies carried out in our laboratory. In this study, the serological activity of MSA was further compared with that of SEAj to evaluate their value in serodiagnosis. The results showed that the sensitivity of both MSA and SEAj for the detection of antibody in patients with schistosomiasis japonica was comparable (91.67%). There was 1.2% false positive rate in normal serum with SEAj, but no positive false reaction was detected with MSA. The amount of antigen consumed in SEAj-ELISA is 2.5 times that consumed in MSA-ELISA, suggesting that MSA has higher serological activity.更多还原