体外培养人垂体腺瘤细胞分泌生长素、催乳素和促性腺素的研究

【摘要】 对40例人垂体腺瘤细胞进行体外培养,研究其合成和分泌GH、PRL、LH、FSH的功能,用放射免疫法测定培养液中激素量,并和血清中含量对照,其中部分病例作电镜对照,发现除PRL腺瘤分泌单一激素外,GH腺瘤部分同时分泌PRL及LH、FSH;无功能性腺瘤经证明大多仍有分泌激素功能。细胞培养方法简易,结果较准确,可以协同其它方法对垂体腺瘤作出更正确而可靠的诊断和分类,并可进行内分泌实验基础及药物治疗机制研究,对了解垂体腺瘤的生物学特性,实现预防和治疗的目的是必不可少的方法之一。更多还原

【Abstract】 Measurement of GH, PRL and FSH, LH have studied with radioimmunoassay in 40 cases of human pituitary tumor cell culcure, and compared with seru mfindings, light and electron microscopy.In this series, all of the 6 prolactinomas showed a high secreting PRL level alone in the culture medium and coincided with serum level as well as the electron microscopy finding. 12 GH adenomas secreted not only GH, but often with PRL, LH and FSH in the culture medium. Furthermore a detectable hormone secretion could also be found in most of the 22 cases of non-functional abenoma, when examined with cell culture.Our work suggested that the method of primary cell culture may be used for:1) Study of human secreting activity of the tumor cell;2) Classification of pituitary adenoma; 3) Collection of some hormones in vitro.更多还原