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细胞色素b5还原酶的提取纯化

THE EXTRACTION AND PURIFICATION OF CYTOCHROME b5 REDUCTASE

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【作者】 王福源;

【Author】 Wang Fuyuan(Shanghai University of Science and Technology)

【机构】 上海科学技术大学;

【摘要】 <正> 肝脏细胞微粒体中的电子传递与类固醇、脂肪酸和药物等的氧化反应有关。细胞色素b5还原酶是细胞色素氧化还原酶系的组成部分之一,存在于细胞的微粒体中,与膜

【Abstract】 A procedure for the extraction and purification of cytochrome b5 reductase frompig liver was described.The results of purification by using two kinds of columnschromatography with DE52-cellulose and Ca10(PO4)6(OH)2 were compared.From theabsorption spectra of enzyme solution and the concentration gradient electrophoresisof polyacrylamide,it was shown that the refined enzyme was rather pure.From 1kg of pig liver,9.2 mg of refined enzyme was obtained with an activity of 22unit/mg.

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