【摘要】 本实验用腹腔注射链佐霉素(Streptozotocin简称STZ)方法破坏小鼠胰岛B细胞以诱发高血糖,观察生长抑素(Somatostatin,SS)、神经降压素(neurotensin,NT)、胰高血糖素(glucagon,GC)和促甲状腺素释放激素(TRH)4种胃肠激素对小鼠高血糖的影响。在每天腹腔注射STZ(60mg/kg)前10分钟分别经皮下注射上述4种胃肠激素,连续注射5天,在实验的第1,6,8,10,15天取血测血清葡萄糖浓度,对照组注射生理盐水(NS)。结果发现:(1)预先注射SS和NT可不同程度地抑制由STZ引起的实验性高血糖,并呈剂量一效应关系,(2)预先注射GC和TRH,血糖浓度仍明显升高,与NS对照组比较无显著差异,(3)取注射STZ后第15天的高血糖小鼠(血糖高于350mg％者)分为8组,分别以SS和NT作治疗性注射,每天一次共5日,并未见对小鼠高血糖有缓解效果;(4)正常小鼠单独皮下注射NS、SS、NT、GC、和TRH,每天一次连续5天,在注射后15天内未见对血糖水平有明显影响。以上结果提示:预防性注射SS和NT可显著抑制由STZ诱发的高血糖的产生。更多还原

【Abstract】 Four gut hormones, somatostatin ( SS ) , neurotensin ( NT ) , glucagon ( GC ) and TRH have been used to observe their effects on the hyperglycemia induced by streptoZotocin ( STZ ) . The changes of serum glucose concentration were assayed by the method of glucose-oxidase. The results were as follows. ( 1 ) Injection of SS or NT 10 minutes prior to STZ could reduce the hyperglycemic level induced by STZ in varying degrees on the day 6th, 8 th 10th, 15th of experiment and showed a dose-related response. However, no change of hyperglycemia induced by STZ has been found after preventive injection of GC or TRH. ( 2 ) There was no reduction of serum glucose concentration, after therapeutic injections of SS or NT into the hyperglycemic (serum glucose 350 mg% ) mice 15 days after STZ treatment. ( 3 ) The concentration of serum glucose was kept at normal levels on normal animals after subcutaneous injections of NS, SS, NT, GC or TRH. These results demonstrate that the injection of SS or NT can prevent the production of hy-perglycemiait induced by STZ, but they do not have therapeutic effect when the hyperglycemia h-as been produced. This suggests that both of these two gut hormones might play a role in the cytoprotection of islet B cells.更多还原