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SPA—血凝试验检查鼠疫半抗体的方法研究

A STUDY OF EXPERIMENTAL METHOD THE SPA-IHA TEST DETECTED INCOMPLETE ANTIBODY OF PLAGUE

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【摘要】 <正> 鼠疫间接血球凝集试验(以下简称血凝试验)虽是目前国内外用于诊断鼠疫 FI 抗体较敏感和特异的方法,但对于鼠疫菌刺激产生的不完全(半)抗体则不甚敏感。面对这一状况,国内鼠疫工作者先后建立和移植了氯化钡检查法、鼠疫 FI 血球抗原中和试验法、血凝—Coombs 试验法,酶联免疫吸附试验和放射免疫法等检查鼠疫半

【Abstract】 A study of experimental method of the SPB-IHA test detdeted incompleteantibody of plague are reported in this paper.The results showed that the test sensitive as much as 9—129 times that inthe IHA test (P<0.001),the geometric average titer (GMT) of 16 referableserum samples was 25.76 times higher than the IHA.The specificity wasvery mighty.The operating method was simple,the experimental propertywas stable and the results might reappear.The using materials and reagentsbasically consisted with IHA test.It is the latest method for detecting inco-mplete anibody of plague and if might generally popularize in the practice.At the same time,the different factors which might effect the action ofSPA were also discussed.Moreover,we have set up the operating methodof SPA—IHA inhibition test.

  • 【文献出处】 中国地方病学杂志 ,Chinese Journal of Endemiology , 编辑部邮箱 ,1985年02期
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