【摘要】 香蕉果实后熟过程中果皮乙烯释放先于果肉组织。果肉磷脂酶活性变化出现最早,呈一峰形升高过程,随后的顺序是细胞膜透性增大,乙烯释放及呼吸跃变。ACC促进及CCCP抑制香蕉果实乙烯的释放表明,其乙烯的形成是通过蛋氨酸途径。 应用CEPA、AOA及CCCP可改变细胞膜透性增大与乙烯释放的先后次序,CEPA使果实乙烯释放的时间提前到与膜透性增大几乎同时发生,经AOA与CCCP处理,细胞膜透性的增大推迟到乙烯高峰出现之后,看来这两者可能不存在因果关系。 1mM ACC可激活未发动后熟香蕉果实果皮及果肉组织乙烯生物合成的机制,CCCP对CEPA诱导释放乙烯的抑制作用表明,CEPA在体内主要通过酶解释放乙烯,CCCP对ACC及CEPA引起的乙烯释放有不同的抑制效应,推测这两者在体内有不同的酶解途径。CCCP未能抑制果皮诱导乙烯释放部分(约占总释放量的20％),可能是CEPA通过非酶途径产生的。更多还原

【Abstract】 During the repining of banana fruits the ethylene released by peel is earlier than that by pulp tissue, and the first change is the phospholi-pase activity rising proceeding has a peak shape and the subsequent or-der is by enhancing the membrane permeability, ethylene release and re-spiratory climacteric. The effects of ACC promotion and CCCP inhibition on ethylene released by banana fruits indicate that ethylene production is by Methionine-Path.CEPA, AOA and CCCP can change the sequence of enhancing cell membrane permeability and ethylene release. CEPA can move up the time of enhancing the membrane permeability almost to the occurrence of ethylene release. The time of enhancing of membrane permeability is de-ferred by AOA and CCCP to come after the appearance of ethylene re-lease peak. It dose not seem that causal relationship exists between the two.1mM ACC can activate the ethylene bio-synthesis mechanism in un-ripening banana peel and pulp tissues. The inhibitory action of CCCP on ethylene released by CEPA shows that the change from CEPA to ethy-lene is an enzymolysis process. CCCP exerts different inhibitory effect on ethylene release induced by ACC and CEPA, suggesting that the two have different path of enzymolysis. CCCP can not completely inhibit the ethylene release induced by CEPA.Probably the uninhibited part of the ethylene released by CEPA (up to about 20%) is by non-enzymolysis path.更多还原