【摘要】 本文介绍一种筛选针对血小板及其它细胞表面抗原的单克隆抗体的酶联免疫吸附法(ELISA)。本方法特异性与敏感性较高,10ng/100ul浓度的抗血小板单克隆抗体即呈明显的阳性反应。与放射免疫法(RIA)同时测定100份杂交瘤培养上清,结果ELISA法55份阳性,RIA法57份阳性,符合率为90%,无统计学差异(P=0.75)。ELISA法OD值与RIA法cpm值之间高度相关(r=0.89)。ELISA简单安全,抗原细胞用量为RIA的1/50,是一种实用的方法。更多还原

【Abstract】 An ELISA method was established for screening monoclonal antigbodies on the surface of platelets and other cells, and was compared with Radioimmunoassay(RIA). 10ng/100ul level of anti-platelet antibody AN51 gave a positive reaction by ELISA method. In 100 samples of hybridoma supernatant, ELISA and RIA gave 55 and 57 positive results respectively, with no statistical difference (P>0.5). The rate of coincidence was 90%. OD value of ELISA and epm of RIA were eorelated(r=0.89). Compared to RIA, ELISA was safer and simpler, and neededs less antigen cells(1/50 of RIA). So, ELISA was a practical method for hybridoma screening test.更多还原