【摘要】 本文报道了人体细胞质胸腺嘧啶核苷激酶(TK-C;EC2.7.1.21)基因的一个片段同TKcDNA的一个片段,一起转化小鼠TK缺陷型细胞株Ltk~-细胞时,这两个单独都不能表达TK活性的DNA片段可能通过同源重组,重新构建成一个能够表达TK的完整的功能单位。分析了TK~+并发转化细胞表达TK的稳定性,并用Southern印迹法杂交证实这两个外源DNA片段已重组成一个片段而整合在受体细胞基因组里,因而并发转化细胞全是稳定型。讨论了两个功能上可以互补的外源DNA片段,在受体细胞里重组成一个能表达基因活性的功能单位的可能机制。更多还原

【Abstract】 A fragment of human cytosolic thymidine kinase (TK-C) gene and a fragment of human TK cDNA were introduced into thymidine kmase-deficient mouse L cells by DMA-mediated gene transfer. Southern blot analyses demonstrated that these two fragments have reconstructed as an intact functional unit expressing TK activity. The mechanism involved may be the homologous recombination. The stability of thymidine kinase-producing transformants was analyzed. All transformants studied express stably TK. The fate of exogenous DNA fragments in host cells was discussed.更多还原