【摘要】 应用植物血凝素（PHA）和脂多糖（LPS）激活淋巴细胞,以氢-胸腺嘧啶核苷（³H-TdR）、碳一尿嘧啶核苷(¹⁴C-UR)和碳-缬氨酸作掺入实验,以分别反映T、B淋巴细胞转化过程中的DNA、RNA和蛋白质的合成能力。共测定了50例肿瘤病人,与正常人比较:T淋巴细胞转化能力明显降低,B淋巴细胞转化能力显著增高。经过一个疗程的⁶⁰Co照射后T细胞转化比照前又显著降低,B细胞转化比照前又显著增高,三种大分子合成都表现同样的规律,反映了辐射抑制T淋巴细胞DNA和RNA合成,相反地刺激了B淋巴细胞的DNA、RNA和蛋白质的合成。以上的辐射效应随照射剂量和照射野的增加而愈益明显。更多还原

【Abstract】 Whole blood was obtained for culture in liquid medium in vitro. Under the stimulation of PHA and LPS respectively, we have used the method of DNA incorporated with 3H-TdB, RNA incorporated with 14C-UR and protein incorporated with 14C-valine to study the synthesis of these three macromolecules during the course of T and B lymphocyte transformation. Tests were carried out in tumor patients before and after radiation therapy with 60Co yrays- the comparison of incorporation has been made between normal subjects and patients before and after radiation therapy. This demonstrated that the y-rays irradiation had a damage effect on DNA and ENA synthesis in T lymphocytes definitely, while it had an enhancing effect on DNA, ENA and protein synthesis in B lymphocytes. Such implies that the cell mediated immunity was impaired while the humoral immune regulation was enhanced in tumor patients exposed to 60Co Y-rays. With increase of radiation dosage and exposure area, the radiation effect increased. The results were discussed briefly.更多还原