【摘要】 我们用急性粒细胞性白血病病人外周血白细胞和小鼠骨髓瘤Ag14—SP2/0细胞（HGPRT^-）为材料,以国产聚乙二醇（分子量10,000）为促融剂,杂交后在含有滋养层细胞的24孔培养板中经HAT培养液选择后获得三个杂交瘤细胞系:XM—H—B₁,XM—H—A₂和XM—H—D₂,经Giemsa—11分化染色,染色体G分带、C分带和G6P—D同功酶的醋酸纤维素薄膜电泳鉴定,证实为人鼠杂交瘤。值得提出的是杂种细胞的上清,经中国医学院科学院基础所测定:①对抗人全血出现沉淀线;②作SDS电泳——PAGE出现克隆带,分子量65,000万左右;③作各种人免疫球蛋白（包括IgG亚类）均阴性,但对一种抗人r血清出现沉淀线,其中XM—H—A₂有两条带。这次获得的三个杂交瘤细胞系说明我们正在建立的杂种细胞分布板是一个很好的材料,同时对于急性粒细胞白血病的发病机制和采用单克隆抗体技术诊断和治疗白血病研究提供了有效手段。更多还原

【Abstract】 Human acute granulocytic leukemia leucocytes and mouse myeloma Ag14—SP2/0（HGPRT^-） were used as parental cells for the fusion experiments in the presence of PEG. The cells were grown on the feed layer cells after being fused. Three hybrid clonse were selected and isolated in the HAT medium: XM—H—B₁, XM—H—A₂, and XM—H—D₂. Giemse—11 differential staining, G—banding, C—banding techniques and isozymes analysis of G6P—D on cellulose acetate membrane electrophoresis were used to identify the above clones. our results showed that clone XM—H—B₁, XM—H—A₂ and XM—H—D₂ are the hybridoma of human and mouse myloma These clones are the useful material for our hybrid clone panel in studing the human gene mapping. Besides, these clones are the further useful tools for studing the pathogenesis of acute granulocytic leukemia and the diagnosis and treatment of the acute granlocytic leukemia.更多还原