【摘要】 对707头牛进行了酶联免疫吸附试验,其中感染血吸虫牛200头,检出率为97.5～97.7％,368头未感染血吸虫牛阴性符合率为97.2～98.5％,对寄生肝片吸虫、双口吸虫、东毕吸虫、棘球蚴牛共81头试验结果仅1头寄生肝片吸虫牛呈阳性反应,80头牛是阴性反应,说明ELISA用于牛血吸虫病诊断具有较高的敏感性与特异性,对基本消灭血吸虫病地区的现场试验也取得相似结果,说明BLISA是一种诊断牛血吸虫病较好的方法。更多还原

【Abstract】 Enzyme-linked immunosorbent assay ( ELSA ) has been developed for the detection of Schistosoma japonica infections in cattle and buffaloes. Seven-hundred-and-seven schistosomefree animals and animals infected with S.japonica were tested. The test has been found to have high sensitivity and specificity. 97.5-97.7% of S.japonica-infected animals were positive,and 97.2-98.5% of schistosome-free animals were negative. Eighty of 81 cattle infected with Fasciola,Paramphi stomum, Orientobilhar-zia and Echinococcus were negative, only one infected with Fasciola was positive. The test conducted in the endemic area also gave good result. The ELISA is not technically difficult to perform and is capable of handling large number of samples.更多还原