【摘要】 本试验采用荧光葡萄球菌A蛋白(FITD-SPA)菌体粘附试验,红细胞(E)花环试验和红细胞抗体(EA)花环试验以及同时采用联合E和EA花环试验对猪外周血液淋巴细胞表面标志进行了研究。凡带有表面膜免疫球蛋白(SmIg)能粘附FITC-SPA菌体和可与免抗鸡红细胞(α-CRBC)抗体包被的CRBC形成花环的淋巴细胞均属于B淋巴细胞类;凡能与绵羊红细胞(SRBC)形成花环者属T淋巴细胞类。 猪外周血液淋巴细胞是采用聚蔗糖-泛影葡胺(比重=1.077克/ml)分层液经梯度离心(2,000rpm)而制备的。 在此研究中共测试了20头健康猪的外周血液淋巴细胞样本,发现带SmIg的淋巴细胞平均为17.29±3.704(范围9.5～26.0)％;形成EA花环的淋巴细胞平均为26.05±2.481(范围23.0～31.0)％;形成E花环的淋巴细胞平均为23.9±2.687(范围19.0～29.0)％。 我们也同时采用联合E和EA花环试验对20头猪的外周血液淋巴细胞样本进行平行测定,结果:EA花环细胞(B)平均为25.65±3.99(范围17.0～30.5)％;E花环细胞(T)平均为24.45±4.365(范围15.0～30.5)％;极少数带有双重受体的淋巴细胞(D)平均为O.575±0.494(范围0.0～1.5)％;余下的为非效应细胞(N)。更多还原

【Abstract】 The membrane surface markers of swine peripheral blood lymphocytes were studied by using of fluorochrome labeled staphylococcus protein A ( FITC-SPA ) adhension test, erythrocyte ( E ) rosette assay, erythocyte antibody ( EA ) rosette assay and combined E and EA rosette assay. Bone marrow dependent ( B ) lymphocytes were characterized by surface immu-noglobulin and Fc receptors which formed EA rosette with antibody-coated chicken erythrocytes. Thymus dependent ( T ) lymphocytes formed nonimmune E rosette with normal sheep erythrocytes.Peripheral blood lymphocytes were separated by density gradient-cen-trifugation in sodium diatrizoute solution ( density 1.077g/ml ) .The mean value of twenty clinically normal swine perpheral lymphocytes are: Ig bearing lymphocytes 17.29 ± 3.704% ( 9.5-26.0% ) ,EA rosette forming cells 26.05±2.481% ( 23.0-31.0% ) and E rosette forming cells 23.9± 2.687% ( 39.0-29.0% ) Combined E and EA rosette assay indicate that the percentage of rosette forming cells are approximate that in separated assay, T cells 24.45±4.365% ( 15.0-30.5% ) ,B cells 25.65±3.99% ( 17.0 -30.5% ) , lymphocytes with both receptors ( D ) are 0.57±0.49% ( 0 -1.5% ) and residues are ineffector cells ( N ) .更多还原