【摘要】 用甘薯“新种花”的丛枝病植株进行茎尖培养,获得7%去除类菌质体的植株。经2年8个月的形态观察、嫁接鉴定、氨基酸分析,证明其均恢复到一般健康植株的外部形态与内部代谢应有的水平。由此可见,象去除病毒一样,茎尖培养同样可以应用于去除类菌质体。试验还表明,以 MS 为基本培养基,附加 IAA0.5毫克/升,或 IAA0.5毫克/升+NAA0.2毫克/升,是作茎尖培养的较好培养基。6BA 促其大块愈伤组织形成,腺素在一定程度上消除6BA不利影响,并有75%外植体长成植株。更多还原

【Abstract】 The production of sweet-potato in Fujian Province is always infectedwith a mycoplasmalike organism which symptoms show that the diseasedplant is little-leaf,chlorosis,stunting and switches-broom.This path-ogen organism is transmitted by leafhoppers and if it infects sweet-patatoplant in the early growing period,the plant will defoliate extensively andwill yield no tubers.So the local growers are anxious for getting goodsweet-potato seedlings with high-yield and mycoplasmalike organism-freecharacters.During past three years we have done a lot of work on sh-oot tip culture and finally these plantlets have been observed carefullyabout their shape-appearances aminoacid contents,and have been testedby graft compared with those diseased plants,7% of these plantletswere assured that they are mycoplasmalike organism-free.We are sure this shoot tip culture method is worth while in product-ion of sweet-potato.Of course,before planting these seedlings the insectvectors leafhoppers must be cleaned off thoroughly.更多还原