【摘要】 分析水稻与高粱杂交后代酯酶同工酶,其酶谱基础与母本粳稻相同,但子代中的种胚、花药等器官出现一条与高粱位置相同而母本水稻没有的第Ⅱ酶带。同时,系统分析了 F₂～F₃及高世代,揭示了这条酶带的遗传现象:杂种各世代多数株系和单株具有这条酶带,并出现分离,绝大多数发生在 F₂～F₃,F₄以后减轻,至高代不再分离;在 F₂～F₅连续世代中,第Ⅱ酶带的出现有七类不同情况。实验结果表明:杂种获得了高粱的遗传物质,导致分子水平的表型变异;异源DNA 在子代中有被排斥、分离、最后稳定的过程;从缺失第Ⅱ酶带的植株后代中又有这条酶带出现,亦可看出表型变异不在于结构基因,而是基因表达调控的结果。更多还原

【Abstract】 The esterase isoenzyme of the hybrids between rice and sorghumhave been analysed,the zymogram of the esterase isoenzyme in hybridsare essentially the same as those of the female plant（the japanese type）.But a No.Ⅱ band in embryo,anther and other organs of hybrids whichdid not appear in maternal plant was found in sorghum.The segregati-ons of this band could be seen is each generations,but appeared mostfrequently in F₂-F₃,decreased in F₄ and steadied on higher generations.There are seven types of segregations found in F₂～F₅.Experimental re-sults show that the hybrids can obtain from sorghum the genetic materi-al,which results in the phenotype variation of the progenies on molec-ular level;this indicares that the heterogenetic DNA obtained by thehybrid undergo a gradual process of exclusion,segregation and final sta-bilization in their progenies.The No.Ⅱ band appears again in the succ-eeding generations of the plants which have lost this band,it also indi-cates that the phenotype variation is taken place because of the control-ling of gene expression,not because of the structure gene.更多还原