【摘要】 本文报告了已成功地获得五株能分泌抗日本血吸虫虫卵主要血清学抗原(MSA)单克隆抗体的小鼠淋巴细胞杂交瘤细胞系NJ85,经体外与在小鼠体内传代与增殖,均能持续地分泌特异性抗体。用酶联免疫吸附试验(ELISA)测定杂交瘤细胞的培养液上清、植瘤小鼠的腹水及其血清,其抗体滴度分别为1:16～128、1:6,400～102,400和1:12,800～204,800。这项工作为提纯MSA以及进一步探索该单克隆抗体在日本血吸虫病的免疫诊断上的应用展示了前景。更多还原

【Abstract】 Five murine hybridoma cell lines secreting monoclonal antibody against the major serological antigen(MSA) of Schistosoma japonicum eggs were produced by fusing NS11 myeloma cells with spleen cells from BALB/c mice that had been immunized with MSA of Schistosoma japonicum eggs. The hyorid cells lines were demonstrated as hybridoma by karyotyping. The hybrid cell line H6 stored in liquid nitrogen can easily be recovered after thawing and still maintain the ability to secrete antibody. The titres of monoclonal antibodies of the mouse ascites induced and the serum of the same mouse detected by the means of ELISA were 1:6400-102400, 1:12800-204800 respectively.更多还原