【摘要】 本文采用¹²⁵碘标记放射免疫方法,以固相二抗作为分离系统,不经萃取,直接快速测定唾液中孕酮及雌二醇的浓度。连续每日观察10名育龄妇女正常月经周期中唾液和血清中孕酮及雌二醇的水平变化。滤泡期、排卵期及黄体中期的唾液中孕酮浓度分别为57.7±11,69.3±10及364±33pmol/L（G.M±S.D）;雌二醇浓度则分别为1.88±0.6,4.46±0.12及2.56±0.3pmol/L（G.M±S.D）。唾液中激素水平约为血清的0.5～3％,整个月经周期中,唾液和血液的二种甾体激素水平起伏一致,呈平行关系,其相关性显著（r>0.64,p<0.001）。大多数受试者在黄体期夜间（24:00～6:00）,唾液的雌二醇水平较高,下午（14:00～16:00）水平较低而平稳。二组间差异显著（P<0.01）。孕酮水平未见明显昼夜变化。唾液孕酮及雌二醇的测定,有标本收集方便的优点,适用于连续测定,可以作为监测卵巢功能及预测排卵的可靠指标。更多还原

【Abstract】 Salivary samples were analyzed for progesterone and estradiol concentrations by the rapid direct （non-extraction）assay, using an ¹²⁵I-tracer and second antibody solid phase as separating system. Daily blood and saliva samples from ten volunteers were measured throughout one menstrual cycle. Levels of progesterone in saliva in the follicular, periovulatory and luteal phases were 57.7±11, 69.3± 10 and 364±33 pmol/L （GM-SD） respectively. The corresponding values for estradiol were 1.88 ±0.6, 4.46 ±0.12 and 2.56 ±0.3 pmol/L. The levels of the steroids in saliva and serum increased and decreased synchronously throughout the whole cycle. There was a significant correlation （p<0.001 for both steroids） between the concentrations in the two body fluids. In most of the seven volunteers, the levels of the salivary estradiol during luteal phase were higher at night （24:00～6:00 hr） and lower and stable in the afternoon （14:00～16:00 hr）. The circadian rhythm, however, was not observed in progesterone. It is apparent that salivary assays are convenient in sample collection, and can be used to indicate ovarian function.更多还原