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炭凝试验诊断猪气喘病的研究
Study on Carbon-agglutination test for the Diagnosis of Swine Enzootic Pneumonia
【摘要】 我们进行了炭凝试验方法的研究,测定了炭末大小,抗原浓度等试验条件,建立了炭凝试验诊断猪气喘病的程序。用这种方法对已知阳性猪及阴性猪进行了检查,并对忻州两个猪场的203头猪进行了检查,检出率为52.7%;间接血凝对照检出率为53.6%。某中一农场的感染率高达88.8%,此外,还检查了某肉联厂的62头待宰猪,50%出现阳性反应,琼扩作为对照,检出43.6%,结果表明,炭凝试验具有敏感性高,特异性强,操作简便,反应快速等优点,适于现场捡疫及大规模普查应用。
【Abstract】 In the course of serological diagnostic study of swine enzootic pneumo- nia,some work has be n done on the carbon-agglutination test.with the definition of the size of carbon particles and the optimum antigenic con- centration to sensitize the carbon,a procedure was developed for the diag- nosis of swine enzootic pneumonia,Using this method,some infected pigs and healthy pigs known were detected.A 52.7% affection rate was detected in 203 pigs from two farms.(in one farm the A.R.is as high as 88.8%).Compared with indirect hemagglutination,53.6% was found.More- over,A.R.50% of the 62 pigs detected from a sloughter house was by carbon-agglutin-ation test.while agar-gel precipitation test only detected antibody in 43.6% of the pigs.As the results show:carbon-agglutination test is a sensitive,specific and simple method for field use.
- 【文献出处】 山西农业大学学报 ,Journal of Shanxi Agricultural University , 编辑部邮箱 ,1985年02期
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