【摘要】 以可见光为作用光照射天然紫膜,紫膜蛋白被280nm紫外光激发所发射的荧光强度比对照略有降低.比较天然紫膜、漂白紫膜与菌蛋白三者的紫外荧光强度,前两者无显著变化,但菌蛋白的荧光强度比天然紫膜的荧光强度大2-3倍,表明生色团对蛋白质荧光可能有猝灭作用.用280nm波长光照射紫膜的暗适应形式,可使其转变成光适应形式.若有羟胺存在,以紫外光照射也可使紫膜漂白.光漂白的作用光谱,其紫外部分与紫膜蛋白部分的吸收光谱重合得很好.上述实验证明紫膜蛋白部分吸收的能量可以转移到生色团上,即紫膜存在分子内的能量转移更多还原

【Abstract】 The UV fluorescence spectra of purple membrane fragments were measured. The fluorescence intensity of protein in the PM samples irradiated with orange light was lower than that of control. The intensities of UV fluorescence of PM and apomembrane were similar, but the quantum yield of UV fluorescence of bacterioopsin was twice as large as that of the natural purple membrane. This result illustrated that the protein fluorescence was possibly quenched by the chromophore of PM. The conversion of the dark-adapted PM into light-adapted PM was induced by irradiation of UV light at 280nm. The photobleaching of PM was also induced by the UV light in the presence of hydroxylamine. By measuring the action spectrum of photobleaching of PM we have showed that the action spectrum of photobleaching of PM coincided with the absorption spectrum of PM in the UV region.All the results showed that the energy absorbed by protein had transferred to retinal.更多还原