【摘要】 本文用5-氮氧自由基硬脂酸、12-氮??氧自由基硬脂酸和16-氮氧自由基硬脂酸三种脂肪酸自旋标记物对人红细胞膜(血影)的不同深度膜脂的流动性作了进一步的研究,所得到的电子自旋共振波谱表明它们的序参数都随γ照射剂量的增大而增大。本文还用马来酰亚胺氮氧自由基标记在膜蛋白的SH基团上,所得到的波谱表明其旋转相关时间和强固定化对弱固定化组分的谱线高度的比值也都随γ辐照剂量增大而增大。结果说明γ辐照后,人红细胞膜的流动性降低。这与我们用荧光探针研究所得到的结果相似。更多还原

【Abstract】 In this communication,threelipid spin labels,methyl 5-doxyl-stearate (5NS),methyl 12-doxylstearate(12NS) and methyl 16-doxylstearate(16NS),were used to investigate thefluidity of lipids in various depths oferythrocyte membranes (ghosts).Itwas found that all their order para-meters increased with increasing ?-raydosages.4-maleimido-2,2,6,6-tetra-methyl piperidino-1-oxyl (MSL) wasused to label the SH group of mem-brane protein.Its rotational corre-lation time and its ratio of the heightsof peaks of strong and weak immobi-lization increased with increasing ?-ray doses.These results showed that?-irradiation can induce a reductionof the fluidity of erythrocyte mem-branes.The same conclusion has beenobtained by us with the aid of thefluorescent probes.更多还原