【摘要】 纯化的柱孢鱼腥藻铁蛋白能够与棕色固氮菌的钼铁蛋白有效地交叉反应,展现较高的活性。此异源交叉反应的乙炔还原比活及放氢比活,分别是蓝藻同源互补比活的83.8及66.7％。比较藻铁蛋白与菌钼铁蛋白异源交叉反应及藻固氮酶组分之间的同源反应的动力学特点时发现,铁蛋白对钼铁蛋白的最佳克分子比数前者(异源交叉反应)较后者(藻同源反应)为高,前者为5,后者为1;但反应的时间进程两者差别不大。更多还原

【Abstract】 A modified method was used for the separation and purification of Anabaena cylindrica nitrogenase, based upon which a further study on the cross-reactivity between Fe-protein from A. cylindrica and MoFe-protein from Azotobacter vinelandii was made. Results indicated that these two heterologous components were able to give an effective cross-reaction and showed a relatively strong nitrogenase activity. The specific activities of acetylene reduction and H₂-evolution contributed by cross-reacting heterologous components were as high as 83.8% and 66.7%, respectively, of that produced by algal homologous complementary response. In comparison with the kinetics of the algal homologous complementary reaction, it was found that the optimum molar ratio of Fe-protein to MoFe-protein for heterologous cross-reaction is much higher （5:1）, the time course for tooth reactions being approximately the same. Discordant with the current conception, our findings demonstrate that the Fe-protein of A. cylindrica can form effective complexes with the MoFe-protem of Azotobacter.更多还原