【摘要】 <正> 在动物饲料中,脂溶性维生素如:维生素A,D₃和胡罗卜素等含量,特别是D₃的含量是非常重要的。存在于饲料中的维生素不仅含量低,而且还有大量干扰物质如:油脂、蛋白质和无机盐类等。所以用高效液相色谱对饲料中的脂溶性维生素进行测定是比较困难的。更多还原

【Abstract】 A procedure is described for the extraction, separation and quantification of vitamin A, D3 and β-carotene in various feeds. At first vitamin A, Ds and β-carotene were extracted by using THF. The extracts were then separated by HPLC with a μ-Bondapak C18 column and a ternary solvent system (THF-CH3CN-H2O) , and quantified with a dual channel UV detector.The quantification by measuring peak height gave wider linear range. The minimum detectable amounts of vitamin A, D3 and β-carotene were 2.9×10-8g, 1.0x10-8g and 1.3×10-9g,and the average recoveries were 80.26%,86.77% and 83.26%, respectively. This method is fast, sensitive and universal.更多还原