【摘要】 <正> 冬凌草甲素(Rubescensin A,以下简称甲素),是从植物冬凌草中经提取分离出的一种抗癌有效成分。它具有多方面的药理作用,目前临床上用于治疗食管癌、肝癌和胰腺癌等。为给临床合理用药提供理论依据,研究甲素的动力学特性,准确测出服药后血液中甲素浓度随时间变化的规律显得十分重要。对药物和生理体液中微量成分的分离和测定,目前大部分都采用高效液相色谱法。但血液中甲素的高效液相色谱测定,国更多还原

【Abstract】 Rubescensia A in blood has been developed by reversed phase high performance liquid chroma-lography using a ALC/GPC-244 apparatus (Wa-ter Associates). The separation was made on a column (300×3.9mmI.D.) packed with Bondapak C18 bonded silica gel using methanol-water as mobile phase.The method involved initial sample concentration with SEP-PAK Cl8 Cartridge. The average percent recovery of Rubescensin A in blood was 88% and the fraction was detected by UV at 254 nm. Minimum detectabls amount of the method is 80ng. By use of this method to study kinetics of Rubescensin A in body better results has been obtained.更多还原