【摘要】 本文研究了应用反相高效液相色谱法分离甜菊糖甙,对其中主要成分甜菊甙进行了鉴定和定量测定。分析条件如下:柱.YWG—C₁₈H₃₇;流动相,甲醇-水（75:25,体积/体积）;紫外检测波长205nm,甜菊甙最小检知量:0.2μg. 分析了18个甜菊糖甙样品,定量测定结果:甜菊甙含量在43.80～60.39％,相对标准偏差为1.6％.更多还原

【Abstract】 The stevia glucosides, extracted from the leaves of stevia rebaudiana Bertoni, is a mixture of glucosides in which the main component is stevioside. In this investigation a reversed phasehigh-performance liquid chromatography (RP-HPLC) was applied to the separation, identification and quantitative determination of stevioside. Analytical conditions for HPLC was as follows; column, YWG-C18H37; mobile phase, methaaol-water (75:25,V/V); ultraviolet detector, 205nm. The limit of detection was 0.2μg of stevioside. 18 samples of glucosides were analyzed. The amounts of stevioside determined were in the range; 43.80% to 60.39%. The relative standard deviation of this method is 1.6%.更多还原