【摘要】 本文报道12株分泌抗乙型肝炎病毒表面抗原单克隆抗体（HBsAg McAb）杂交瘤细胞的建株及其 McAb 性质的分析结果,用 ID、CEP、PHA 和 ELISA 等四种方法均测得较高水平的McAb。杂交瘤细胞培养液中 McAb 的 PHA 效价为1:80～1:320,由杂交瘤诱生的小鼠腹水 McAb的 ID 和 CEP 效价可高达1:3,200～6,400,PHA 和 ELISA 滴度分别为10^-4～10^-5和10^-5～5×10^-7。将这些腹水以 PBS 稀释到 10^-5,分别与¹²⁵I-HBsAg（2×10⁴cpm）结合,其结合率为52.8～68.4%.12个 McAb 的特异性均良好,其中1个为抗“HBs/d”亚型抗体,其余的均为抗“HBs/a”型特异性抗体。这些 McAb 均属小鼠 IgG₁亚类。选出其中3个 McAb 用于制备 HBsAg 诊断试剂,获得良好结果。更多还原

【Abstract】 The establishment of 12 strains of hybridomas secreting anti-HBsAg-McAb and thecharacterization of these McAb are described.The significant antibody levels weredetected by four methods （ID,CEP,PHA,ELISA）.The PHA titers in the supernateof the hybrid cell culture were 1:80 to 1:320.In the mice ascitic fluid derived fromanti-HBsAg hybrid cells,the titers of McAb in ID and CEP were as high as 1:3.20,200to 1:6,400.The titers in PHA and ELISA ranged 10^-4 to 10^-6 and 10^-5 to 5×10^-7.respectively.The avidity of these McAb was 52.8%-68.4% when the ascitic fluid wasdiluted with PBS to 10^-5 and detected by ¹²⁵I-HBsAg（2×10⁴ cpm）binding assay.It wasfound that only one of these 12 strains of hybridomas was of“anti-HBs/d” sub-type,the others were“anti-HBs/a”type.All these McAb belonged to mice IgG₁ sub-class.Three strains of these hybridomas have been selected to prepare the diagnostic reagentsfor HBsAg.更多还原