【摘要】 本文报道用高效液相色谱法测定消炎痛（Indomethacin）血浓度的方法和结果。高效液相色谱仪填以反相YWG-C₁₈（10μm）,并带有254nm UV检测器。流动相为甲醇∶水∶乙腈∶冰醋酸（63∶27∶10∶0.1ml,V/V）,流速为1ml/min。内标物选用炔诺酮。消炎痛的保留时间t_R=9min,内标物t_R=6min10s。线性范围为0.1～5.0μg/ml血清,检测限为0.05μg消炎痛。应用本法测定7名健康受试者的消炎痛血清浓度,其药代动力学参数平均值为:K=0.1677h^-1,K_α=2.4597h^-1,t_1/2=4.44h,C_max=2.3924μg/ml,t_max=1.65h,AUC_0～12h=6.7525μg.h/ml。结果表明,本法简便快速,适于临床进行消炎痛血清浓度的监测。更多还原

【Abstract】 A method of high performance liquid chromatography (HPLC) for quantitative determination of indomethacin in serum is described. Chromatography was carried out on a model YSB liquid chromatography fitted with a reversed-phase YWG-C18 particle size 10 μm column and equipped with 254 nm UV detector. The mobile phase used was a mixture of methanol: water: acetonitrile: glacial acetic acid (63:27:10:0.1v/v) and the flow rate was1ml/min. Norethindrone was used as an internal standard. The retention times for indomethacin and norethindrone were 9’and 6’10" respectively. The method was linear for concentration from 0.1 μg-5 μg/ml serum. The detection limit was 0.05 μg. Each of seven healthy volunteers were given one dose of 250 ing indomethacin orally. The serum concentration was determined by the above HPLO method. The pharmacokinetic parameters of indomethacin obtained are as follows (mean value) :K= 0.1677 h-1, Ka=2.4597h-1, T1/2=4.44h, Cmax=2.3924μg/ml, Tmax=1.66h, AYC0-12h = 6.7525μg.h/ml. The HPLO system described is rapid, precise and accurate for clinical monitoring of indomethacin concentrations in serum.更多还原