【摘要】 本文报道一种测定人血浆游离脯氨酸的简便方法。用纸层析法分离,以正丁醇:冰醋酸:乙醇:水(5:1:2:1)为展开剂,吲哚醌试液为显色剂,生成蓝色化合物于分光光度计608±1nm处测定其吸光度,通过标准曲线计算其含量。用本法测得正常人血浆游离脯氨酸量为235.4±74.4μmol/L(n=36),晚期血吸虫病人为107.8±39.3μmol/L(n=22),两者有显著差异(p<0.01)。本法专一性强,回收率高(97.47％),检出限为0.5μg,所用仪器设备简单,适合于临床检验用。更多还原

【Abstract】 A simple and specific method for estimation of free proline in human plasma was recommended in this paper. The proline was separated completely from other amino acids with n-butanol-aoetie aoid-ethanol-water(5:1:2:1) as mobile phase by paper chromatography. The chromatograms were run overnight for a total of 30 hr. at room temperature. They were sprayed with isatin and heated at 80℃ for 30 min, and then a dark blue spot was manifested. The excess of isatin was washed with water at 40℃ for 5 min, and then the chromatographio paper was dried at 60℃. The blue spot was cut off and put into 5 ml phenol saturated with water. Eluted for 13 min, it was determinated at 608℃1nm by UV-speotrophotometer.It was found that the mean plasma concentration of free proline in normal subjects as 235,4×74.4 μmol/l (n = 36) and 107.8×39.3μmol/l in late stage of schistosomiasis (n = 22). An apparent difference was observed between normal subjects and patients (p<0.01).This method has advantages of good specificity, high recovery (mean value 97.47%) and simple experimental apparatus; therefore, the method is particularly adapted to clinical diagnoses of hepatic cirrhosis.更多还原