【摘要】 本文用抗HBs/a McAb腹水用亲和层析法有效地提纯了HBsAg,回收率较高;并进行了CIEP检测临床血清标本,沉淀线清晰,检测灵敏度高于抗HBsAg血清。将抗HBs/a和抗HBs/d二种McAb提纯后制备诊断血球,进行RPHA检测临床血清标本千余例,并经美国Abbott实验室RIA试剂盒验证,表明其阳性检出率与中和试验符合率较国内目前以PcAb制备的诊断血球为优,显示了良好的特异性、灵敏度和准确性。抗HBsAgMcAb可作为新一代的HBsAg检测试剂。更多还原

【Abstract】 Twelve hybridoma cell lines secreting anti-HBsAg McAb have been established in our laboratory. HBsAg in serum or plasma has been effectively purified by the ascitic anti-HBs/a McAb affinity chromatography, and its recovery rate was higher. When clinical specimens were detected by the ascitic anti-HBs/a McAb CIEP, the sensitivity was higher than that by sheep anti-HBs PcAb serum.Sensitized RBC for HBsAg diagnosis were prepared using RBC coupled with a mixture of purified anti-HBs/d and anti-HBs/a McAbs. RPHA test was used to screen more than 1000 specimens and the data obtained from part of those specimens were reconfirmed by AUSRIA Ⅱ-125 radioimmunoassay (Abbott Laboratories).The results show that the percentage of positive specimens detected and the accordance rate with neutralization assay by McAb diagnostic RBC were superior to that by PcAb diagnostic RBC which was made at home, and the specificity, sensitivity as well as the accuracy of McAb diagnostic RBC in RPHA were fine.We conclude that the anti-HBs McAb can be used as a new generation reagent for the detection of HBsAg.更多还原