【摘要】 <正> 本文报道了,用细胞核移植的方法,获得亲缘关系更远的,不同亚科鱼类之间的核质杂种鱼,即由鲤科(Cyprinidae)中,雅罗鱼亚科(Leucinae)的草鱼(Ctenopharyngodon idellus)的胚胎细胞核和鳊(?)亚科(Abramidinae)的团头鲂(Megalobrama amblycephala)的卵细胞质配合而成的核质杂种鱼。被移植的卵子共659个,得杂种成鱼24尾,成活率为3.6%。更多还原

【Abstract】 Nucleo-cytoplasmic hybrid fish are obtained from the combination of nucleus and cytoplasm from two subfamilies of fresh-water teleosts using the technique of nuclear transplantation,i.e. the combination of the nucleus of grass carp (Ctenopharyngoden idellas) and the cytoplasm of blunt-snout bream (Megalobrama amblycephala). The average survival rate of the hybrid fish is 3.6%.Some morphological characteristics of those nucleo-cytoplasmic hybrid fish(head in body length, depth in body length, width in body length, dorsal spine, abdominal keel, anal rays, gillracker, pharyngeal teeth, leteral line scales and number of vertebrae)are similar to those of grass carp. it indicate that the expression of genetic information related to those characteristics of nucleo-cytoplasmic hybrid fish were influenced by the donor nucleus of grass carp.Analysis of the chromosome patterns of grass carp, blunt-snout bream and the nucleo-cytoplasmic hybrid fish shows that their chromosome numbers are all the same (2n=48) and most chromosomes in above three kinds of fish are difficult to be distinguished morphologically, However, priliminary examination shows that the eleventh pair of subtelocentric chromosomes are somewhat different in grass carp and blunt-snout bream which we used in our experiments: the arm-ratio of the eleventh pair of chromosomes in grass carp is>3, and that in blunt-snout bream is 2. Examination also confirms that the arm-ratio of the eleventh pair of chromosomes of nucleo-cytoplasmic hybrid fish is>3. Evidently, the nucleus of nucleo-cytoplasmic hybrid fish is obtained from grass carp cell by nuclear transplantation.Preliminary observation shows that the growth rate of the nucleo-cytoplasmic hybrid fish is much faster than that of blunt-snout bream and also a little faster than that of grass carp. None of the nucleo-cytoplasmic hybrid fish was found to be dead of infective disease which is always observed in grass carp.So far, one of the nucleo-cytoplasmic hybrid male fish has matured and produce normal sperms which could be used to fertilize the grass carp eggs. In view of the fact that in sexual hybridization between remote species of fish such as that in grass carp and blunt-snout bream, their hybrids are always abnormal, lethal at early embryonic stages or sterile (due to the poor development of their gonads)even few of them survived, it is clear that the method of nuclear transplantation as an useful biotechnique can be used for obtaining the nucleo-cytoplasmic hybrid fish clones. As well as to provide a possibility in forming new breeds of edible fish. The further study of this nucleo-cytoplasmic hybrid fish will be reproted in seperate paper elsewhere.更多还原